Flow cytometry is a process used to analyze cell characteristics. Using a laser and fluorescently tagged proteins parameters such as cell size health and phenotype can be determined.
Flow cytometry is a standard laser based technology that is used in the detection and measurement of physical and chemical characteristics of cells or particles in a heterogeneous fluid mixture.
What is flow cytometry. What is flow cytometry. Using this method multiple parameters of single cells can be analyzed. Flow cytometry is a laboratory method used to detect identify and count specific cells.
From wikipedia the free encyclopedia flow cytometry fc is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. What is flow cytometry. Flow cytometry is a semi quantitative technique that allows you to analyze the frequency and other properties of cells stained with specific fluorochrome conjugated antibodies.
Flow cytometry is used to determine the physical and chemical properties of cells in a heterogeneous population. Flow cytometers fc or fcm are automated instruments that quantitate properties of single cells one cell at a time. Flow cytometry is a high speed technology by which single cells cell aggregates or small multicellular organisms in liquid suspension can be classified and viably sorted at real time rates well in excess of thousands per second.
Flow cytometry is so refined that individual proteins can be tracked. Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. They can measure cell size cell granularity the amounts of cell components such as total dna newly synthesized dna gene expression as the amount messenger rna for a particular gene amounts of specific surface receptors amounts.
In this process a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. This method can also identify particular components within cells. Cell classification by flow cytometry departs from automated microscopy in that cells are identified by a minimal set of scalar parameters without attempting to spatially resolve and analyze images.
This information is based on physical characteristics and or markers called antigens on the cell surface or within cells that are unique to that cell type.